Used as a fluorescent dye in biological staining and microscopy to visualize cell structures, particularly in tracing neuronal pathways and studying membrane permeability. Its fluorescence properties make it suitable for use in tracer studies, where it helps monitor the movement of molecules in tissues or across barriers. Also applied in ophthalmic diagnostics to assess corneal integrity and tear film dynamics due to its ability to bind to damaged epithelial surfaces. The compound’s high sensitivity under UV light enhances contrast in imaging, supporting detailed cellular analysis.