Used as a high-performance fluorescent dye in bioimaging and cellular staining due to its strong photostability and bright emission in the visible range. Its lipophilic nature allows easy penetration into cell membranes, making it suitable for live-cell imaging. Commonly employed in fluorescence microscopy and flow cytometry for tracking intracellular processes. Also utilized in the development of sensors for detecting specific ions or environmental changes within biological systems. The acetate groups enable enzyme-triggered activation in targeted areas, enhancing specificity in diagnostic applications.