Used primarily as a fluorescent tracer in medical diagnostics, especially in ophthalmology for angiography to visualize blood flow in the retina and choroid. Helps detect retinal vascular abnormalities such as leakage, blockages, or neovascularization. Also employed in intraoperative imaging to assess tissue perfusion during surgeries. Its fluorescence properties enable real-time imaging under blue light, improving precision in diagnosis and surgical guidance.