Used primarily as a fluorescent tracer in biological and medical research, enabling visualization of cellular structures and monitoring of dynamic processes in live cells. Its lipophilic nature allows it to integrate into lipid membranes, making it useful for cell membrane labeling and tracking membrane dynamics. Commonly employed in fluorescence microscopy and flow cytometry due to its strong green fluorescence upon excitation. Also utilized in diagnostic applications, such as detecting corneal abrasions in ophthalmology when activated by blue light. Stable in lipid environments, it serves as a marker in drug delivery systems to trace the distribution of lipid-based carriers.