6-FITC is widely used as a fluorescent labeling agent in biological and biomedical research. Its primary application lies in fluorescence microscopy, where it is conjugated to antibodies, proteins, peptides, or nucleic acids to enable visualization of cellular components and molecular targets. Due to its high fluorescence quantum yield and stability, 6-FITC is effective in flow cytometry for cell surface marker analysis and cell sorting. It is also employed in enzyme-linked immunosorbent assays (ELISA) and other immunoassay techniques to detect antigen-antibody interactions. The dye’s excitation and emission properties make it compatible with standard fluorescence detection equipment, such as argon-ion lasers and fluorescence microscopes equipped with FITC filter sets. Additionally, 6-FITC is used in live-cell imaging and receptor binding studies, offering reliable performance in tracking molecular dynamics in real time.