Widely used in biological research as a fluorescent labeling agent, it enables the detection and visualization of specific molecules within cells and tissues. It binds to proteins, antibodies, or nucleic acids, allowing researchers to track their location and movement under fluorescence microscopy. Commonly applied in immunofluorescence, flow cytometry, and histochemistry to study cellular structures and molecular interactions. Its bright green fluorescence makes it ideal for multiplex assays when combined with other fluorophores. Also used in medical diagnostics to assess corneal injuries and tear film dynamics in ophthalmology.