Widely used in biological labeling and detection due to its strong green fluorescence under UV light. Commonly conjugated to antibodies, proteins, or nucleic acids for use in fluorescence microscopy, flow cytometry, and immunohistochemistry. Enables real-time tracking of cellular components and molecular interactions. Also applied in ELISA and other assay systems for signal amplification. Stable and compatible with standard filter sets, making it a preferred choice in multiplex staining when combined with other fluorophores.