Used in bioorthogonal chemistry for labeling biomolecules in live cells without interfering with natural biological processes. Commonly applied in fluorescence imaging to track biomolecules such as proteins and glycans. Enables site-specific conjugation in peptide and protein modifications due to its selective reactivity with alkynes. Frequently employed in drug discovery and development for tagging and detecting target molecules in complex biological environments. Suitable for click chemistry reactions in aqueous conditions, making it ideal for cellular and in vivo applications.