Widely used as a fluorescent labeling reagent in biological and medical research. It binds to proteins, antibodies, or other biomolecules through its isothiocyanate group, enabling detection and visualization under fluorescence microscopy. Commonly applied in immunofluorescence techniques to identify specific antigens in cells or tissues. Also used in flow cytometry for cell surface marker analysis and in enzyme immunoassays as a tracer. Its bright green fluorescence makes it suitable for real-time tracking of molecular interactions in live-cell imaging. Additionally employed in ophthalmology to diagnose corneal injuries and study tear film dynamics due to its high visibility and water solubility.