Fluorescein5(6)-isothiocyanate
90%, 5- and 6-isomer mixture
Reagent
Code: #127131
Alias
Fluorescein isothiocyanate; 5(6)-fluorescein isothiocyanate, 4'(or 5')-fluorane isothiocyanate
CAS Number
27072-45-3
science Other reagents with same CAS 27072-45-3
blur_circular Chemical Specifications
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Molecular Information
Weight
389.38 g/mol
Formula
C₂₁H₁₁NO₅S
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Registry Numbers
EC Number
248-207-7
MDL Number
MFCD00005063
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Physical Properties
Melting Point
>360 °C(lit.)
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Storage & Handling
Storage
2-8°C
description Product Description
Used extensively as a fluorescent labeling reagent in biochemistry and molecular biology for tagging proteins, antibodies, and other biomolecules. It enables visualization and tracking of these molecules in techniques like fluorescence microscopy, flow cytometry, and immunoassays. Its isothiocyanate group reacts with primary amines, forming stable thiourea bonds for covalent attachment. Commonly applied in diagnostics, cellular imaging, and research to study molecular interactions, localization, and dynamics in biological systems.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Appearance | Light yellow to yellow to orange to brown powder to crystal |
| Purity (%) | 89.5-100 |
| Infrared Spectrum | Conforms To Structure |
| NMR | Conforms to Structure |
| Solubility (1 mg/mL, Acetone) | Very Faint Yellow To Yellow And Very Faint Green-Yellow To Green-Yellow Liquid |
| Carbon (C) | 54.1-71.0 |
| Nitrogen (N) | 3.0-4.0 |
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