Sulfonfluorescein is widely used as a fluorescent tracer in various scientific and industrial applications. Its strong fluorescence properties make it ideal for tracking and monitoring fluid flow in environmental studies, such as groundwater movement and hydrological investigations. In biochemistry and molecular biology, it serves as a fluorescent marker for labeling biomolecules, enabling visualization and analysis in techniques like fluorescence microscopy and flow cytometry. Additionally, it is employed in medical research for imaging and diagnostic purposes, particularly in studying cellular processes and tissue dynamics. Its water solubility and stability under diverse conditions further enhance its utility in experimental and analytical procedures.