5-Aminofluorescein is widely used as a fluorescent tracer in biological and chemical research. Its high fluorescence intensity makes it ideal for labeling and tracking molecules in various experimental setups. In cell biology, it is employed to study cell permeability, membrane dynamics, and intracellular transport processes. It is also utilized in fluorescence microscopy to visualize cellular structures and monitor live-cell activities. Additionally, 5-Aminofluorescein is applied in flow cytometry for cell sorting and analysis. In biochemistry, it serves as a probe for enzyme activity assays and protein interactions. Its compatibility with aqueous solutions and stability under physiological conditions further enhance its utility in diverse scientific applications.