Resorufin sodium salt is widely used in biochemical and cellular research as a fluorescent indicator. It is particularly valuable in assays that measure enzymatic activity, such as those involving oxidoreductase enzymes. The compound’s ability to emit strong fluorescence upon reduction makes it a key component in viability and cytotoxicity assays, where it helps determine cell health and metabolic activity. Additionally, it is employed in environmental monitoring to detect the presence of specific pollutants or toxins, leveraging its sensitivity to redox reactions. In diagnostic applications, resorufin sodium salt is used in the development of biosensors for detecting biomarkers associated with diseases. Its versatility and reliability in fluorescence-based detection systems make it a staple in both research and industrial settings.