SF7-AM is widely used in biological research as a fluorescent probe for detecting hydrogen sulfide (H₂S) in living cells. It is particularly valuable for studying H₂S signaling pathways and its role in physiological and pathological processes. The acetoxymethyl ester group allows it to penetrate cell membranes easily, where intracellular esterases hydrolyze it to release the active fluorescent form. This enables real-time imaging and quantification of H₂S levels in live cells and tissues, making it a critical tool in neuroscience, cardiovascular research, and studies of oxidative stress. Its high sensitivity and selectivity make it a preferred choice for exploring H₂S-related mechanisms in various biological systems.