4-O-Methylhelichrysetin is used in molecular biology research due to its potential anti-inflammatory and antioxidant properties. It inhibits enzymes involved in inflammatory processes, such as cyclooxygenase-2 (COX-2), and reduces the production of pro-inflammatory cytokines, making it a promising candidate for development as an active ingredient in health products or treatments for chronic inflammatory diseases, such as arthritis or diabetes. Preliminary studies suggest it may exhibit anti-proliferative effects on certain cancer cells, particularly liver and breast cancer cells, but research is still in the in vitro and animal testing stages, with no clinical use yet.

In cosmetic formulations, it is valued for its skin-brightening properties, helping to reduce the appearance of dark spots and even skin tone by inhibiting melanin production. Its antioxidant activity makes it suitable for anti-aging products, protecting skin from free radical damage. The anti-inflammatory effects soothe sensitive or irritated skin. Due to its stability and compatibility with other active ingredients, it is commonly included in serums, creams, and lotions targeting hyperpigmentation and photoaging.