Lumiflavin, a derivative of vitamin B2 (riboflavin), is primarily utilized in biochemical research as a fluorescent probe due to its inherent fluorescence properties. It is often employed in studies involving flavoproteins and flavin-dependent enzymes to understand their structure, function, and interaction mechanisms. In photochemistry, lumiflavin serves as a model compound to investigate the photochemical behavior of flavins, aiding in the study of light-induced reactions and electron transfer processes. Additionally, it is used in analytical chemistry as a standard or reference material for the quantification and identification of flavin derivatives in various samples. Its role in studying oxidative stress and redox reactions also makes it valuable in biomedical research, particularly in exploring cellular processes related to metabolism and signaling pathways.