Mdivi-1 is primarily used in research settings to study mitochondrial dynamics. It works by inhibiting Drp1, a protein involved in mitochondrial fission, which helps researchers understand the role of mitochondrial division in cellular processes. This compound is particularly valuable in studying diseases where mitochondrial dysfunction is a key factor, such as neurodegenerative disorders, cardiovascular diseases, and cancer. By preventing excessive mitochondrial fragmentation, Mdivi-1 aids in exploring potential therapeutic strategies for conditions linked to impaired mitochondrial function. Additionally, it is used to investigate the impact of mitochondrial dynamics on apoptosis, providing insights into cell survival and death mechanisms.