Ferrostatin-1 (Fer-1) is primarily used as a potent inhibitor of ferroptosis, a form of regulated cell death driven by iron-dependent lipid peroxidation. Its applications span various fields of biomedical research, particularly in studying and mitigating cell death in diseases where ferroptosis plays a critical role. In neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, Fer-1 is employed to protect neurons from ferroptosis-induced damage, offering potential therapeutic insights. It is also utilized in research on ischemia-reperfusion injury, where ferroptosis contributes to tissue damage during the restoration of blood flow after ischemia. Additionally, Fer-1 is applied in cancer research to explore the role of ferroptosis in tumor suppression and to investigate its potential as a target for cancer therapy. It is also used in studies of acute organ injuries, such as acute kidney or liver injury, where ferroptosis is implicated in the pathological process. Overall, Fer-1 serves as a valuable tool in understanding the mechanisms of ferroptosis and developing strategies to combat related diseases.