FASN-IN-3 is primarily used in research settings to study the role of fatty acid synthase (FASN) in various biological processes. It is applied as a potent inhibitor of FASN, which is an enzyme involved in the synthesis of fatty acids. By inhibiting FASN, researchers can investigate its impact on cancer cell growth and proliferation, as many cancer cells rely heavily on fatty acid synthesis for survival. This compound is particularly useful in exploring potential therapeutic strategies for cancers that exhibit upregulated FASN activity. Additionally, it aids in understanding metabolic pathways and their connection to diseases such as obesity and diabetes, where fatty acid metabolism plays a critical role.