3-hydroxy lignoceric acid finds its application primarily in the field of biochemistry and medical research. It is used as a biomarker for the diagnosis of certain genetic disorders, particularly those related to lipid metabolism such as adrenoleukodystrophy (ALD). In ALD, the accumulation of very long-chain fatty acids, including 3-hydroxy lignoceric acid, in the body's tissues and fluids is a key diagnostic indicator. Researchers also utilize this compound in studies focused on understanding the mechanisms of fatty acid oxidation and the role of peroxisomes in lipid metabolism. Additionally, it serves as a reference standard in analytical chemistry for the quantification of fatty acids in biological samples, aiding in the development of diagnostic assays and therapeutic monitoring.