Triacsin C is primarily used in biochemical research to study lipid metabolism, particularly the processes involving acyl-CoA synthetases. It effectively inhibits these enzymes, which are crucial for converting fatty acids into their active form, acyl-CoA. This inhibition allows researchers to explore the role of fatty acids in cellular energy production, signaling pathways, and lipid storage. Additionally, Triacsin C is utilized in studies related to lipid droplet formation, fatty acid oxidation, and the regulation of lipid-related diseases such as obesity and diabetes. Its application extends to investigating the impact of lipid metabolism on cancer cell survival and proliferation, providing insights into potential therapeutic targets.