Methyl pentadecanoate is primarily used in the research and development of biofuels. It serves as a model compound in studies aimed at optimizing biodiesel production processes, particularly in understanding the esterification and transesterification reactions. Its properties make it suitable for analyzing the efficiency of catalysts and reaction conditions in converting fatty acids and triglycerides into biodiesel.

In the field of organic chemistry, it is employed as a standard or reference material in gas chromatography and mass spectrometry for the identification and quantification of fatty acid methyl esters. This is crucial in analyzing the composition of complex mixtures, such as those found in vegetable oils, animal fats, and other lipid sources.

Additionally, methyl pentadecanoate finds application in the synthesis of specialty chemicals and intermediates. It is used in the production of surfactants, lubricants, and plasticizers, where its long hydrocarbon chain contributes to desired physical and chemical properties. Its role in creating biodegradable and environmentally friendly products is also of interest in sustainable chemistry research.