Methyl heneicosanoate is primarily used in research and analytical chemistry as a standard or reference compound in gas chromatography and mass spectrometry. Its well-defined properties make it suitable for calibrating instruments and ensuring accurate measurements of fatty acid methyl esters. In the field of lipidomics, it aids in the identification and quantification of complex lipid mixtures, contributing to studies on metabolic pathways and lipid profiles. Additionally, it serves as a model compound in the synthesis and study of long-chain esters, which are important in the development of biofuels, lubricants, and specialty chemicals. Its applications extend to the cosmetic and pharmaceutical industries, where it may be used in the formulation of emollients or as an intermediate in the production of bioactive molecules.