Used as a fluorogenic substrate in enzymatic assays, particularly for detecting esterase activity. Upon enzymatic cleavage, it releases resorufin, a highly fluorescent compound, enabling sensitive detection in biological samples. Commonly applied in high-throughput screening, cell-based assays, and real-time monitoring of gene expression. Also utilized in environmental testing to assess microbial activity in samples. Its low background fluorescence and strong signal output make it ideal for detection in complex matrices.