FTO-IN-2 is primarily utilized in research focused on targeting fat mass and obesity-associated protein (FTO), an enzyme linked to obesity and metabolic disorders. It serves as a potent inhibitor of FTO, making it valuable in studies exploring the regulation of fat storage, energy balance, and obesity-related pathways. Researchers employ FTO-IN-2 to investigate its potential therapeutic applications in treating obesity, type 2 diabetes, and other metabolic syndromes. Additionally, it is used to study the role of FTO in epigenetic modifications, particularly in RNA demethylation processes, which influence gene expression and cellular functions. Its application extends to drug discovery efforts aimed at developing novel treatments for metabolic diseases.