PFI-3 is primarily utilized in biochemical research as a potent and selective inhibitor of the BRPF (Bromodomain and PHD Finger-containing) family of proteins, particularly targeting BRPF1, BRPF2, and BRPF3. Its application is significant in studying the role of these proteins in chromatin remodeling and gene regulation. Researchers employ PFI-3 to investigate its effects on epigenetic pathways, particularly in the context of cancer and other diseases where BRPF proteins are implicated. By inhibiting BRPF proteins, PFI-3 helps in understanding their biological functions and potential as therapeutic targets. Additionally, it is used in cell-based assays and in vitro studies to explore its impact on histone acetylation and transcriptional regulation, providing insights into the development of novel epigenetic therapies.