PFI-2 is primarily used in biochemical research as a selective inhibitor of the SETD7 (SET domain-containing protein 7) enzyme, which is involved in the methylation of histone and non-histone proteins. This compound is valuable in studying the role of SETD7 in various cellular processes, including gene expression regulation, cell cycle control, and signal transduction pathways. Researchers utilize PFI-2 to explore its potential implications in diseases such as cancer, where SETD7 activity may contribute to tumor progression or suppression. By inhibiting SETD7, PFI-2 helps elucidate the enzyme's function and its impact on cellular mechanisms, aiding in the development of targeted therapeutic strategies.