Used primarily in clinical diagnostics, where it catalyzes the hydrolysis of creatinine to creatine and ammonia, facilitating the measurement of creatinine levels in blood and urine. This enzyme is essential for assessing kidney function, as elevated creatinine levels often indicate impaired renal activity. It is also employed in biosensors and diagnostic kits for quick and accurate detection of kidney-related disorders. Additionally, it finds application in research settings for studying metabolic pathways involving creatinine.