Phospholipase C, Phosphatidylinositol specific, from Bacillus cereus, is widely used in biochemical research to study cell signaling pathways. It specifically hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) into inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), which are critical secondary messengers in cellular processes. This enzyme is instrumental in investigating the role of the phosphoinositide pathway in various physiological functions, including calcium mobilization, protein kinase C activation, and cell proliferation. Additionally, it is employed in the preparation of membrane fractions for lipid analysis and in the development of assays to measure phospholipase activity. Its specificity and efficiency make it a valuable tool in understanding membrane dynamics and signaling mechanisms in both normal and diseased states.