This enzyme is widely used in biochemical research and diagnostic applications due to its ability to catalyze the hydrolysis of inorganic pyrophosphate into orthophosphate. It plays a crucial role in DNA and RNA synthesis processes, where it helps to drive reactions forward by removing pyrophosphate, a byproduct of nucleotide polymerization. In molecular biology, it is often employed to enhance the efficiency of PCR and other enzymatic reactions by preventing pyrophosphate accumulation, which can inhibit polymerase activity. Additionally, it is utilized in studies of energy metabolism and phosphate regulation, providing insights into cellular processes. Its application in industrial biotechnology includes improving the yield of certain bioprocesses by controlling pyrophosphate levels.