L-Glutamine Synthetase from Escherichia coli is widely utilized in biochemical research to study enzyme kinetics and regulation mechanisms. It plays a critical role in the biosynthesis of glutamine, making it essential for investigating nitrogen metabolism in cells. The enzyme is also employed in the development of biosensors for detecting ammonia and other nitrogen-containing compounds. Additionally, it is used in industrial biotechnology for the production of glutamine and related amino acids in fermentation processes. Its application extends to the study of neurodegenerative diseases, where glutamine metabolism is a key focus area.