Used primarily in biochemical research to study the Calvin cycle, where it plays a critical role in carbon fixation during photosynthesis. It is employed to understand enzyme kinetics, catalytic mechanisms, and the efficiency of carbon dioxide assimilation in plants. Additionally, it serves as a model enzyme for exploring protein structure-function relationships and for developing strategies to enhance photosynthetic efficiency in agricultural crops. Its applications also extend to environmental studies, particularly in assessing the impact of climate change on plant physiology.