Phosphodiesterase I from Crotalus adamanteus venom is widely used in biochemical research for its ability to hydrolyze phosphodiester bonds in nucleic acids. This enzyme is particularly valuable in studies involving DNA and RNA, where it aids in the analysis of nucleotide sequences and structures. It is employed in the preparation of nucleic acid samples for sequencing and in the investigation of nucleic acid-protein interactions. Additionally, it is utilized in enzymatic assays to study the mechanisms of phosphodiesterase activity and to develop inhibitors for therapeutic purposes. Its specificity and efficiency make it a crucial tool in molecular biology and genetic research.