Chymotrypsinogen A is primarily used in biochemical research and medical applications. In research, it serves as a precursor to chymotrypsin, an enzyme that breaks down proteins, making it valuable for studying protein digestion and enzyme kinetics. In medicine, it is utilized in therapeutic treatments, particularly in wound care and inflammation reduction, where its activated form, chymotrypsin, helps to degrade damaged tissue and promote healing. Additionally, it is employed in certain diagnostic procedures to analyze protein structures and functions. Its role in facilitating protein breakdown also makes it useful in industrial processes, such as the production of protein hydrolysates for nutritional supplements.