This solution is widely used in cell culture laboratories for detaching adherent cells from the surface of culture vessels. It is particularly effective in breaking down proteins that facilitate cell adhesion, allowing cells to be harvested without significant damage. The inclusion of EDTA enhances the action by chelating calcium and magnesium ions, which are essential for cell-to-surface attachment. This makes it a valuable tool in processes such as subculturing, cell passaging, and preparing cells for downstream applications like flow cytometry or cryopreservation. Its gentle yet efficient action ensures high cell viability and integrity, making it a preferred choice for maintaining healthy cell lines.