Peroxidase horseradish (POD) is widely used in biochemical assays, particularly in enzyme-linked immunosorbent assays (ELISAs), for detecting and quantifying specific proteins or antibodies. It catalyzes the oxidation of substrates, producing a detectable color change, which is essential for visualizing results in diagnostic tests.

In molecular biology, POD is employed in immunohistochemistry to label and identify specific antigens in tissue samples, aiding in disease diagnosis and research. It is also utilized in biosensors for detecting hydrogen peroxide and other analytes, leveraging its high specificity and catalytic efficiency.

Additionally, POD plays a role in environmental monitoring, where it is used to detect pollutants and toxins in water and soil samples. Its ability to amplify signals makes it valuable in low-concentration detection scenarios.

In the food industry, it is applied to measure glucose levels and ensure product quality, particularly in dairy and beverage production. Its versatility and reliability make it a critical tool across various scientific and industrial fields.