DPN Nucleosidase is used in biochemical and molecular biology research to cleave nicotinamide nucleosides, particularly hydrolyzing NAD+ (nicotinamide adenine dinucleotide) into nicotinamide and a nucleotide. This aids in the study of NAD+ metabolism, energy metabolism pathways, DNA repair, and related enzymatic processes. It enables control of NAD+ levels in experimental systems, facilitating analysis of coenzyme structures, degradation products, and their impacts on cellular processes such as sirtuin protein function and cellular aging. Additionally, it is employed in biosensor systems to detect NAD+ levels or activities of NAD+-related enzymes in biological samples.