Widely used in molecular biology and diagnostics, this enzyme plays a key role in removing phosphate groups from nucleic acids and proteins, facilitating processes like DNA cloning and labeling. In clinical testing, its activity in blood serves as a biomarker for liver and bone disorders, helping diagnose conditions such as hepatitis or osteomalacia. It is also employed in biotechnology for dephosphorylation of vectors to prevent self-ligation during recombinant DNA work. Additionally, it finds use in some immunoassays as a detection enzyme due to its stable and detectable activity.