Diisopropyl-fluorophosphatase is primarily used in the detoxification of organophosphorus compounds, which include many pesticides and nerve agents. It breaks down toxic substances like diisopropyl fluorophosphate (DFP) and related chemicals by hydrolyzing the phosphorus-fluorine or phosphorus-oxygen bonds, rendering them less harmful. This makes the enzyme valuable in medical and environmental applications, particularly in developing antidotes for organophosphate poisoning. It is also studied for use in decontamination solutions for skin, equipment, or surfaces exposed to nerve agents. In biotechnology, it serves as a model enzyme for understanding enzyme catalysis and designing improved bioscavengers for chemical defense.