N6-Etheno NAD is primarily utilized in biochemical research as a fluorescent analog of NAD (nicotinamide adenine dinucleotide). Its application is significant in studying enzyme mechanisms, particularly those involving NAD-dependent enzymes. The etheno modification introduces fluorescence, allowing researchers to monitor enzyme activity and binding interactions in real-time. This makes it a valuable tool in understanding metabolic pathways, such as glycolysis and the citric acid cycle, where NAD plays a crucial role. Additionally, it aids in investigating the dynamics of protein-ligand interactions, providing insights into the structural and functional aspects of enzymes. Its use extends to drug discovery, where it helps in identifying and characterizing inhibitors of NAD-dependent enzymes, contributing to the development of therapeutic agents.