2-Naphthyl β-D-glucopyranoside is used in biochemical research as a chromogenic substrate for studying enzyme activity, particularly for β-glucosidase and other glycosidase enzymes. Upon hydrolysis by these enzymes, it releases 2-naphthol, which can be easily detected by spectrophotometry, aiding in the quantitative assay of enzyme activity. It helps in understanding the mechanisms of glycoside hydrolysis. Also employed in the synthesis of complex carbohydrates and glycoconjugates. Applied in the development of diagnostic assays to detect enzyme deficiencies. Utilized in the food industry to enhance flavor profiles by breaking down glycosides into aromatic compounds. Plays a role in pharmaceutical research for drug delivery systems involving glycosidic bonds. Additionally, it is used in studies of carbohydrate metabolism and cellular processes related to glycosidase function.