This chromogenic substrate is widely used in biochemical research and diagnostic assays to study enzyme activity, particularly for proteases like trypsin and thrombin. It serves as a specific substrate that, when cleaved by the target enzyme, releases a colored product (p-nitroaniline), allowing for the quantification of enzyme activity through spectrophotometric analysis. Its application is crucial in understanding enzyme kinetics, inhibitor screening, and the development of therapeutic agents. Additionally, it is employed in clinical diagnostics to detect and measure protease levels in biological samples, aiding in the diagnosis of diseases related to abnormal protease activity.