Used as a fluorescent probe in biochemical assays, particularly for detecting enzyme activity such as β-glucuronidase. The compound serves as a substrate that, upon enzymatic cleavage, releases a fluorescent product enabling real-time monitoring of enzymatic reactions. It is also employed in cellular imaging due to its ability to penetrate cell membranes and generate detectable signals upon activation. Its nitrobenzyl group acts as a photolabile protecting group in controlled release studies, making it useful in photochemical applications and drug delivery research.