Used as a fluorogenic substrate in enzymatic assays to detect and quantify β-mannosidase activity. Upon enzymatic hydrolysis, it releases 4-methylumbelliferone, a highly fluorescent compound that can be easily measured using fluorescence detection methods. This makes it valuable in biochemical and cellular research for studying enzyme kinetics, screening inhibitors, and diagnosing lysosomal storage disorders related to β-mannosidase deficiency. Commonly used in high-throughput screening and clinical diagnostics due to its sensitivity and specificity.