NA-MUG is widely used in biochemical research as a substrate for enzyme activity assays, particularly for detecting and quantifying β-glucuronidase activity. Its main application lies in diagnostic tests where enzymatic hydrolysis of NA-MUG releases a fluorescent product, enabling sensitive detection in both clinical and environmental samples. It is commonly employed in microbiology to identify bacterial species that produce β-glucuronidase, such as certain strains of E. coli. Due to its high specificity and measurable fluorescence output, NA-MUG is also utilized in high-throughput screening and cellular assays where real-time monitoring of enzyme expression is required.