Used as a substrate in enzymatic assays, particularly for measuring alkaline phosphatase activity in biochemical and clinical diagnostics. It plays a key role in cell culture media, supporting phosphate-dependent cellular processes in osteoblast differentiation and bone mineralization studies. Commonly included in western blotting and protein phosphorylation experiments to inhibit phosphatases and preserve phosphorylated protein states. Also utilized in enzymatic synthesis and phosphorylation reactions in research settings.