Used in high-throughput screening assays to evaluate the activity of the CYP3A4 enzyme, a major cytochrome P450 isoform involved in drug metabolism. The substrate enables real-time monitoring of enzyme activity by producing a fluorescent signal upon metabolic conversion, allowing researchers to assess drug-drug interaction potential, enzyme inhibition, or induction in pharmaceutical development. Commonly applied in liver microsome studies and recombinant enzyme systems for rapid and sensitive detection without requiring complex sample preparation.