Widely used in molecular biology and biochemistry as a chromogenic substrate for alkaline phosphatase detection. When dephosphorylated by alkaline phosphatase, it produces a blue-colored insoluble precipitate at the site of enzyme activity. Commonly applied in techniques such as Western blotting, ELISA, immunohistochemistry, and nucleic acid hybridization assays to visualize target molecules. Its high sensitivity and low background make it ideal for detection in both membrane-based and solution-based assays. Often used in combination with other substrates for dual-labeling experiments.