BET-IN-1 is primarily utilized in biochemical research as a potent and selective inhibitor of BET (bromodomain and extra-terminal) proteins. These proteins play a critical role in regulating gene expression by binding to acetylated lysine residues on histones. By inhibiting BET proteins, it helps researchers study their function in various cellular processes, including transcription regulation, cell cycle progression, and chromatin remodeling. This compound is particularly valuable in cancer research, as BET proteins are often implicated in the development and progression of certain cancers. It is also used to explore potential therapeutic strategies targeting BET proteins in diseases such as inflammation, cardiovascular disorders, and neurological conditions. Its application extends to drug discovery efforts aimed at developing novel BET inhibitors for clinical use.