Acid Ceramidase-IN-1 is primarily used in research settings to study the role of acid ceramidase in various biological processes. It is a potent inhibitor of acid ceramidase, an enzyme that plays a critical role in the metabolism of ceramides, which are important lipid molecules involved in cell signaling and apoptosis. By inhibiting this enzyme, researchers can investigate its impact on diseases such as cancer, where ceramide levels are often dysregulated. Additionally, it is used to explore potential therapeutic strategies for conditions like Farber disease, a rare genetic disorder caused by acid ceramidase deficiency. Its application extends to understanding the enzyme's role in inflammation, neurodegenerative diseases, and other pathologies linked to ceramide metabolism.